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DMEM无糖培养基 90113
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≥1 ¥220元/瓶
  • 供货能力:1000
  • 最低订购量:1瓶
  • 可销售总数量:1000瓶
  • 建议零销价:220元/瓶
  • 发布日期: 2019-11-29
  • 更新日期: 2019-11-29
产品详细说明
品牌: Solarbio CAS编号:
产地: 北京 英文名称: DMEM
保质期: 1年 货号: 90113
含量: 现询客服 用途范围: DMEM培养基
规格: 500ml 是否进口:

不含葡萄糖、丙酮酸钠、酚红、HEPES,含谷氨酰胺。

DMEM是现今细胞培养中比较常见的培养基之一,dmem培养基是建立在MEM培养基的基础上研究出来的,它与MEM培养基相比较,DMEM培养基中加大了MEM培养基中各种成分的用量,下文主要介绍一下自己配制DMEM的方法。

1 制备新鲜三蒸水或Millipore超纯水。

2 称取所需量的干粉培养基,加入约终体积一半的三蒸水中;若配制一个包装的培养液,在将整个包装的干粉倒入三蒸水后,需用水洗包装袋内面2次,倒入培养液中,以保证所有干粉都溶解成培养液。磁力搅拌或人工搅拌使之完全溶解。

3 根据包装袋上的要求补加所需量的碳酸氢钠;根据实验需要,添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物质。

4 加水定容到终体积。

5 必要时用1 mol/L 盐酸和1 mol/L 氢氧化钠调节pH。

6 用无菌0.22um滤膜过滤除菌,分装于无菌血清瓶中,4℃冰箱保存。

配制好的培养液用前加入100U/mL青霉素和100U/mL链霉素,并根据需要加入血清(5%-20%)。 DMEM培养基应用广泛。基于DMEM培养基中氨基酸、微生物含量丰富,并具有糖酵解中的丙酮酸和微量Fe离子,因此在细胞培养、疫苗生产等方面的应用最为常见。

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