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Caspase-3活性测定试剂盒 BC3830 细胞凋亡检测
起批量( 价格
≥1 ¥780元/盒
  • 供货能力:100
  • 最低订购量:1盒
  • 可销售总数量:100盒
  • 建议零销价:780元/盒
  • 发布日期: 2019-12-16
  • 更新日期: 2020-03-05
产品详细说明
品牌: Solarbio CAS编号:
产地: 北京 货号: BC3830
是否进口: 纯度: 现询客服 %
用途: 细胞凋亡检测 产品规格: 20T

Caspase-3是凋亡过程中最主要的终末剪切酶,因而也是研究最多的caspase,它激活pro-caspase-2,6,7,9等,特异水解多种凋亡关键蛋白如PARP,介导细胞核染色质固缩、凋亡小体生成、核DNA断裂。试剂盒测定原理基于Caspase-3特异水解多肽底物acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA),释放硝基苯胺pNA。游离硝基苯胺pNA呈黄色在405 nm具有*吸收峰,用普通可见光分光光度比色方法测定。根据pNA标准管吸光度OD值和标准曲线,计算来自底物肽释放的pNA浓度,得到Caspase-3水解活性。反应体系100微升,试剂盒提供的试剂,除标准曲线外可进行50或100次样品测定。

适用:测定哺乳动物组织、细胞caspase-3活性。

组成与储存:
1. 裂解缓冲液: 20 ml ,4 oC
2. 反应缓冲液: 10 ml ,4 oC
3. 2 mM Ac-DEVD-pNA底物: 0.5 ml,-20 oC避光
4. 10 mM pNA标准品: 0.5 ml,-20 oC避光
以上标出的量为100次检测,50次检测的试剂量减半。试剂常温运输。到达后按要求储存,1年内稳定。

所需仪器:
酶标仪与96孔酶标板;或可见光分光光度计配100μl容积的石英比色杯。
工作波长:*吸收波长405 nm,如不具备也可在400~450 nm范围内测定。

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