产品简介

  1.名  称：DAB显色试剂盒（20×）

  2.组  成：

 3.货  号：DA1010

  4.品  牌：solarbio

  5.储存条件：20℃避光密闭保存，一年有效。避免反复冻融。短期可2-8℃保存。

  6.简  介：

    二氨基联苯胺（Diaminobenzidine,DAB）是过氧化物酶（POD）的底物，DAB显色液主要用于免疫过氧化物酶法，适用于辣根过氧化物酶HRP标记的免疫印记或免疫组化反应。过氧化物酶使底物发生反应，于反应部位产生棕色沉淀物。免疫印记可直接在膜上显示条带。免疫组化标本显色时间一般为室温5-20分钟，随后在显微镜下观察显色情况，显色充分后应将标本及时脱水封固。其终产物可直接在光镜下观察，也可经OsO4处理后，增加反应产物的电子密度，用于电镜观察。 

 7.注意事项：

  1）．DAB溶液应低温密封保存。如有结晶析出，应确保结晶完全溶解再行使用；

  2）．显色工作液应现用现配，新鲜配制的工作液应为无色或浅棕色，如颜色过深，请勿使用；

  3）．显色时间严格控制，根据情况调整，以免显色过度。固相膜显色数小时后即会褪色，不能*存在；

  4）．DAB为可疑致癌物，请采取必要的防范措施。操作时应戴手套，尽量避免与皮肤接触，用后及时彻底冲洗，接触DAB的实验用品好经洗液浸泡24h后使用。

相关文献：

《Ras-association domain family 1 (RASSF1A) gene regulates progression, migration and invasion of bladder cancer》 作者:Yuhai Bao，XuefengLiu，Yang Liu，Si Wang，Bin Wu 期刊:Surgical Oncology 影响因子:2.136 PMID:
《Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway》 作者:Hongming Zhang, Moyan Liu, Yuyan Zhang, and Xiaoyan Li 期刊:Frontiers in Pharmacology 影响因子:3.845 PMID:30890937
《Overexpression of Forkhead box C1 attenuates oxidative stress, inflammation and apoptosis in chronic obstructive pulmonary disease》 作者:ShuyueXiaaJianQubHuiJiaaWeiHeaJingLibLongZhaoaMingqingMaoaYanZhaoa 期刊:Life Sciences  Pages:75-84 影响因子:3.333 PMID:30428305
《HMGB1-mediated differential response on hippocampal neurotransmitter disorder and neuroinflammation in adolescent male and female mice following cold exposure》 作者:BinXua1Shu-chengZanga1Shi-ZeLiaJing-RuGuoaJian-FaWangaDiWangaLi-PingZhangbHuan-MinYangaShuaiLian 期刊:Brain, Behavior, and Immunity 影响因子:6.306 PMID:30476565
《ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways》 作者:Wei Li Daguang Wang Xuan Sun Yang Zhang Lei Wang Jian Suo 期刊:International Journal of Molecular Medicine   Pages: 914-926 影响因子:2.784 PMID:30569104
《α?lipoic acid protects against carbon tetrachloride?induced liver cirrhosis through the suppression of the TGF?β/Smad3 pathway and autophagy》 作者:Guangwei Liu Jiangkai Liu Linping Pian Songlin Gui Baoping Lu 期刊:Molecular Medicine Reports    Pages: 841-850 影响因子:1.922 PMID:30535447
《Activation of the MAPK signaling pathway induces upregulation of pro-apoptotic proteins in the hippocampi of cold stressed adolescent mice》 作者:BinXua1ShuaiLiana1Jing-RuGuoaJian-FaWangaLi-PingZhangbShi-ZeLiaHuan-MinYang 期刊:Neuroscience Letters 影响因子:2.159 PMID:30711527
《MicroRNA-150 relieves vascular remodeling and fibrosis in hypoxia-induced pulmonary hypertension》 作者:YingLiWeidongRenXinWangXiaonaYuLiCuiXinyangLiXintongZhangBoShi 期刊:Biomedicine & Pharmacotherapy 影响因子:3.457 PMID:30551428
《MicroRNA-210 alleviates oxidative stress-associated cardiomyocyte apoptosis by regulating BNIP3》 作者:Hongying Diao, Bin Liu, Yongfeng Shi, Chunli Song, Ziyuan Guo, Ning Liu 期刊:Bioscience 影响因子:5.876 PMID:28661226
《Lysophosphatidic acid enhances neointimal hyperplasia following vascular injury through modulating proliferation, autophagy, inflammation and oxidative stress》 作者:Xuhui Shen Jianjun Zou Fuyong Li Tianhe Zhang Tongqi Guo 期刊:Molecular Medicine Reports 影响因子:1.922 PMID:
《High Spy1 expression predicts poor prognosis in colorectal cancer》 作者:Qin Jin, Gang Liu, Luri Bao,Yuzhen Ma,Huidong Qi,ZhizhongYun,Yanfeng Dai, and Shu Zhang 期刊:Cancer Management and Research 影响因子:3.702 PMID:30147372
《Catgut implantation at acupoints increases the expression of glutamate aspartate transporter and glial glutamate transporter-1 in the brain of rats with spasticity after stroke》 作者:Rui-Qing Li, Ming-Yue Wan, Jing Shi, Hui-Ling Wang, Fei-Lai Liu, Cheng-Mei Liu,Jin Huang, Ren-Chao Liu, Le Ma, and Xiao-Dong Feng 期刊:Neural Regeneration Research. 影响因子:2.234 PMID:29926828
《The CXCL12–CXCR4 axis promotes migration, invasiveness, and EMT in human papillary thyroid carcinoma B-CPAP cells via NF-κB signaling》 作者:Yuanqiang Lin,Qingjie Ma，Lin Li,Hui Wang 期刊:Biochemistry and Cell Biology 影响因子:2.25 PMID:29316404
《Effects of ELL-associated factor 2 on ultraviolet radiation-induced cataract formation in mice》 作者:Yanhua Jiang, Rongrong Fu, Jiangyue Zhao, Di Wu, Guang Qiao, Ruoxi Li, Jinsong Zhang 期刊:Molecular Medicine Reports 影响因子:1.922 PMID:26328919
《Protein kinase C inhibitor chelerythrine attenuates partial unilateral ureteral obstruction induced kidney injury in neonatal rats》 作者:Bo Shi，Shixing Li，Hao Ju，Xin Liu，Dan Li，Ying Li 期刊:Life Sciences 影响因子:3.448 PMID:30439378
《Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction》 作者:Authors: Jin Zhao Meixia Meng Jinhua Zhang Lili Li Xiaojing Zhu Li Zhang Chang Wang Ming Gao 期刊:Molecular Medicine Reports 影响因子:1.851 PMID:30816496
《High kinesin family member 11 expression predicts poor prognosis in patients with clear cell renal cell carcinoma》 作者:Qin Jin, Yanfeng Dai, Yan Wang, Shu Zhang, Gang Liu 期刊:Journal of clinical pathology 影响因子:2.346 PMID:30819726
《MicroRNA‐181a regulates the activation of the NLRP3 inflammatory pathway by targeting MEK1 in THP‐1 macrophages stimulated by ox‐LDL》 作者:Jinyang Song ， Shaonan Yang ， Ruihua Yin ， Qi Xiao ， Aijun Ma ， Xudong Pan 期刊:Journal of Cellular Biochemistry banner 影响因子:3.448 PMID:30938884
《Nur77 promotes cigarette smoke?induced autophagic cell death by increasing the dissociation of Bcl2 from Beclin-1》 作者:Huiping Qin, Feng Gao, Yanni Wang ,Bin Huang, Ling Peng, Biwen Mo, Changming Wang 期刊:International Journal of Molecular Medicine 影响因子:2.928 PMID:31115481

公司简介

    北京索莱宝科技有限公司始创于2000年，是一家集产品研发、生产、销售、服务于一体的大型高科技生物企业。 公司总部位于北京，拥有1200平米研发生产中心，公司提供的产品近万种，超过5000种常备现货，涵盖分子生物学、细胞生物学、免疫学、生物医学等相关领域，并不断推陈新出扩充种类，为用户提供优秀，专业的产品。 立足北京，辐射全国，随着产品营销战略的不断延伸，我们将逐步发展成为专业性生物科技公司。我们希望能够和尊敬的客户一起，利用各自的资源优势和勇于进取的敬业精神，为中国生命科学研究的发展作出更多的贡献。为了更好、更全面的发展，现公司正在诚招各地代理商，欢迎垂询并期待您的加入！