产品简介：

本公司生产的SDS-PAGE凝胶制备试剂盒是按照经典方法配制而成。本试剂盒提供了配制SDS-PAGE凝胶所需的各种试剂，用户只需自备制胶器具和蒸馏水，即可配制PAGE胶。本试剂盒可配制不同规格、不同聚合度的PAGE胶(即聚丙烯酰胺凝胶) 约25/50块。

注意事项：

1. PAGE胶促凝剂和10%PAGE胶凝固剂*加，在加入前需混匀前面所加试剂。10%PAGE胶凝固剂溶液在4℃有效期为一周； PAGE胶促凝剂易挥发，使用后请盖紧瓶盖。在常温下胶30分钟内可以凝固，如温度过低，可放37℃温箱凝固。灌完分离胶后，轻轻的加1mL ddH2O封上层，胶凝固后可见到分界线。灌浓缩胶前，先倾去水层，再用吸水纸吸干。浓缩胶灌好后立刻插入梳子。浓缩胶凝固后，放入电泳液中(让电泳液漫过加样孔)，轻轻的拨出梳子，可防加样孔变形。

2. 配制量可按上表等比加减。如果所用胶浓度与上面不同，可自行调整，主要是调整30% 制胶液的量（需要浓度×总体积/30%），*用水补足总体积。

配胶说明：

1. 先在PAGE胶凝固剂干粉中加入蒸馏水或去离子水(每克PAGE胶凝固剂需加水10mL)配置成10%溶液，将溶液分装成小体积后冻存于-20℃，制备凝胶时融化后使用。4℃保存有效期为一周。检测SDS溶液是否澄清，若出现絮状物或沉淀可放37℃片刻至溶解，不影响效果。

2. 根据目标蛋白分子量大小，选取凝胶浓度，按下表配制。先配分离胶，再配浓缩胶。

参考文献：

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《Maresin1 regulates neutrophil recruitment and IL-10 expression in Aspergillus fumigatus keratitis》 作者:Qing Tang,Chengye Che,Jing Lin,Hong He,Wenyi Zhao,Leyu Lv,Guiqiu Zhao 期刊:International Immunopharmacology 影响因子:1.638 PMID:30690344

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《Total flavonoid extract of Epimedium herb increases the peak bone mass of young rats involving enhanced activation of the AC10/cAMP/PKA/CREB pathway》 作者:Hui-Rong Xia, Hui-Ping Ma,Fang-Fang Yang, Yu-Hai Gao,Jian Zhou, Yuan-Yuan Wang, Wen-Yuan Li 期刊:Journal of Ethnopharmacology 影响因子:3.414 PMID:29783019

《Proanthocyanidins Protect against β-Hydroxybutyrate-Induced Oxidative Damage in Bovine Endometrial Cells》 作者:Xi Cheng 1,?OrcID, Shuhua Yang 1,?, Chuang Xu 2, Lanzhi Li 1, Yi Zhang 1, Yang Guo 1, Cai Zhang 3, Peng Li 1,* , Miao Long 1,* OrcID and Jianbin He 5,* 期刊:Molecules 影响因子:3.098 PMID:30678309

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《Therapeutic effects of 1,25-dihydroxyvitamin D3 on diabetes-induced liver complications in a rat model》 作者:Lina Liu, Guodong Lv, Conghua Ning, Ye Yang, Jun Zhu 期刊:Experimental and Therapeutic Medicine 影响因子:1.41 PMID:27284312

"《Purification of the insecticidal Cry2Ad protein

from a Bt-isolated BRC-HZP10 strain and

its toxin assay to the diamondback moth,

Plutella xylostella (L.)》 作者:J.Y. Liao, Y.Q. Gao, Q.Y. Wu, Y.C. Zhu and M.S. You 期刊:Genetics and Molecular Research 影响因子:0.58 PMID:26214446"

《A deuterohemin peptide protects a transgenic Caenorhabditis elegans model of Alzheimer’s disease by inhibiting Aβ1–42 aggregation》 作者:Jia Xu,Ye Yuan,Ruining Zhang,Yanhui Song,Tianzhuo Sui,Jiaqi Wang,Chonghan Wang,Yujia Chen,Shuwen Guan,Liping Wang 期刊:Bioorganic Chemistry 影响因子:3.926 PMID:30428413

《Plant-Based Nanoparticles Prepared from Proteins and Phospholipids Consisting of a Core–Multilayer-Shell Structure: Fabrication, Stability, and Foamability》 作者:Xing Chen，Yan Chen，Liqiang Zou，Xingcai Zhang，Yuqing Dong，Jizhou Tang，David Julian McClements，Wei Liu 期刊:Journal of Agricultural and Food Chemistry 影响因子:3.571 PMID:31117503

《Effects of ultrasonics combined with far infrared or microwave thawing on protein denaturation and moisture migration of Sciaenops ocellatus (red drum)》 作者:Luyun Cai，Wendi Zhang，Ailing Cao，Minjie Cao，Jianrong Li 期刊:Ultrasonics Sonochemistry 影响因子:7.279 PMID:31084796

《Synergistic protection of matrine and lycopene against lipopolysaccharide?induced acute lung injury in mice》 作者:Wu?Wei Li， Tao?Yuan Wang ，Bo Cao， Bin Liu， Yu?Mei Rong ，Juan?Juan Wang ，Fei Wei ，Lu?Qing Wei， Hong Chen ，Yan?Xia Liu 期刊:Molecular Medicine Reports 影响因子:1.851 PMID:31180535