BCA蛋白浓度测定试剂盒(500T) PC0020
  • 品牌:Solarbio
  • 产地:中国
  • 型号:500微孔(50T)
  • 货号:PC0020
  • 价格: ¥询价
  • 发布日期: 2020-04-16
  • 更新日期: 2024-03-29
产品详请
产地 中国
品牌 Solarbio
货号 PC0020
保存条件 BSA附件-20℃,其它试剂RT
用途范围 蛋白浓度测定
规格 500微孔(50T)
保质期 1年
是否进口


BCA蛋白浓度测定试剂盒

储存条件:BSA附件-20℃,其它试剂RT,有效期1年

英文名称:BCA Protein Assay Kit



产品简介

BCA蛋白浓度测定试剂盒组成

产品简介:

碱性条件下,蛋白将Cu2+还原为Cu+, Cu+与BCA试剂形成紫蓝色的络合物,测定其在562nm处的吸收值,并与标准曲线对比,即可计算待测蛋白的浓度。常用浓度的去垢剂SDS,Triton X-100,Tween不影响检测结果,但受螯合剂(EDTA, EGTA)、还原剂(DTT,巯基乙醇)和脂类的影响。实验中,若发现样品稀释液或裂解液本身背景值较高,可试用Bradford蛋白浓度测定试剂盒。

操作说明:

一. 微孔酶标仪法

1. 配制工作液:根据标准品和样品数量,按50体积BCA试剂加1体积Cu试剂(50:1)配制成BCA工作液,充分混匀(混合时可能会有浑浊,但混匀后就会消失)。BCA工作液室温24小时内稳定。

2. 稀释标准品:取10μL BSA标准品用PBS稀释至100μL(样品一般可用PBS稀释),使终浓度为0.5mg/mL。将标准品按0, 2, 4, 6, 8, 12, 16, 20μL加到96孔板的蛋白标准品孔中,加PBS补足至20μL。

3. 将样品作适当稀释( 多做几个梯度,如作2倍、4倍、8倍稀释),加20μL到96孔板的样品孔中。由于移液器在取小量样品时误差偏大,标准线前面的点可能不很准确,所以尽可能的让样品点落在标准线1/2后。

4. 各孔加入200μL BCA工作液,37℃放置15-30分钟。用酶标仪测定A562nm,根据标准曲线计算出蛋白浓度。使用温箱孵育时,应注意防止因水分蒸发影响检测结果。

二. 分光光度计法 如没有酶标仪,可用分光光度计在离心管中混匀后加入比色皿中比色。 步骤如下:

1. 配制工作液: 根据标准品和样品数量,按50体积BCA试剂加1体积Cu试剂(50:1)配制成BCA工作液,充分混匀(混合时可能会有浑浊,但混匀后就会消失)。BCA工作液室温24小时内稳定。

2. 稀释标准品:取100μL BSA标准品用PBS稀释至1mL(样品一般可用PBS稀释),使终浓度为0.5mg/mL。

3. 取八支(或者更多)5mL离心管,标上号,按下表加入试剂。

BCA蛋白浓度测定试剂盒实验数据

4.37℃放置15-30分钟。用分光光度计测562nm处吸光值,根据标准曲线计算出蛋白浓度。

注意事项:

1. 长期不用时,Cu试剂与PBS稀释液可置于2-8℃保存,如发现细菌污染则应丢弃。BCA试剂在低温条件下出现结晶沉淀时,可37℃温育使其完全溶解, 不影响使用。

2. 样品中若含有较多干扰物质时,请采用Bradford蛋白浓度测定试剂盒。

3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。


备注:
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.




参考文献:

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《Salvanic acid B inhibits myocardial fibrosis through regulating TGF-β1/Smad signaling pathway》 作者:Hongyan Gao,Zhe Bo,Qin Wang,Ling Luo,Haiyi Zhu,Yi Ren 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:30553195

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《Cetuximab and Doxorubicin loaded dextran-coated Fe3O4 magnetic nanoparticles as novel targeted nanocarriers for non-small cell lung cancer》 作者:Qinlu Zhang,Qian Liu,Menghan Du,Alphons Vermorken,Yali Cui,Lixia Zhang,Lili Guo,Le Ma,Mingwei Chen 期刊:Journal of Magnetism and Magnetic Materials 影响因子:2.683 PMID:

《HDAC2, but not HDAC1, regulates Kv1.2 expression to mediate neuropathic pain in CCI rats》 作者:Zhihua Li, Yanyan Guo, Xiuhua Ren, Lina Rong, Minjie Huang, Jing Cao, Weidong Zang 期刊:Neuroscience 影响因子:3.244 PMID:31022463

《Overexpression of SENP1 reduces the stemness capacity of osteosarcoma stem cells and increases their sensitivity to HSVtk/GCV》 作者:Fengting Liu, Lili Li, Yanxia Li, Xiaofang Ma, Xiyun Bian, Xiaozhi Liu, Guowen Wang, Dianying Zhang 期刊:International Journal of Oncology 影响因子:3.571 PMID:

《Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture》 作者:Yi-Meng Duan, Ying Jin, Meng-Li Guo, Leng-Xin Duan, and Jian-Gang Wang 期刊:Journal of Cancer 影响因子:3.182 PMID:30087713

《Minor structural modifications to Pracinostat produce big changes in its biological responses》 作者:Rong Jia, Pengju Sun, Yan Zhang, Youjin Ge, Niefang Yu 期刊:Chemical Biology & Drug Design 影响因子:2.256 PMID:30932330

《KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells》 作者:Xu Liu,Xiao-Zhong Guo,Hong-Yu Li,Jiang Chen 期刊:Hepatobiliary & Pancreatic Diseases International 影响因子:1.576 PMID:30948327

《Total flavonoid extract of Epimedium herb increases the peak bone mass of young rats involving enhanced activation of the AC10/cAMP/PKA/CREB pathway》 作者:Hui-Rong Xia, Hui-Ping Ma,Fang-Fang Yang, Yu-Hai Gao,Jian Zhou, Yuan-Yuan Wang, Wen-Yuan Li 期刊:Journal of Ethnopharmacology 影响因子:3.414 PMID:29783019

《MicroRNA?26b acts as an antioncogene and prognostic factor in cervical cancer》 作者:Lihong Wang, Wen Wang, Yuanyuan Wu 期刊:Oncology Letters 影响因子:1.871 PMID:30867779

《Proanthocyanidins Protect against β-Hydroxybutyrate-Induced Oxidative Damage in Bovine Endometrial Cells》 作者:Xi Cheng 1,?OrcID, Shuhua Yang 1,?, Chuang Xu 2, Lanzhi Li 1, Yi Zhang 1, Yang Guo 1, Cai Zhang 3, Peng Li 1,* , Miao Long 1,* OrcID and Jianbin He 4,* 期刊:Molecules 影响因子:3.098 PMID:30678309

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《Molecular characterization and expression analysis of protein enhancer of sevenless 2B from Artemia sinica at early embryonic development and during immune response to bacterial stimulation》 作者:YuXiaKangHeLiliJiaoYuanyuanGengDaochuanZhang 期刊:Fish & Shellfish Immunology 影响因子:3.185 PMID:30711491

《Portable Smartphone-based Colorimetric Analyzer with Enhanced Gold Nanoparticles for On-site Tests of Seafood Safety》 作者:Longjie ZHONG, Jiadi SUN, Ying GAN, Shuqi ZHOU, Zijian WAN, Quchao ZOU, Kaiqi SU, Ping WANG 期刊:Analytical Sciences 影响因子:1.355 PMID:30745510

《Immunomodulatory effects of Alhagi honey polysaccharides encapsulated into PLGA nanoparticles》 作者:AdelijiangWusimanabShuwenXuabHaiyuNiabPengfeiGuabZhenguangLiuabYueZhangabTianxinQiuabYuanliangHuabJiaguoLiuabYiWuabDeyunWangabYuLuc 期刊:Carbohydrate Polymers 影响因子:5.158 PMID:30824082

《Lower dosage of aspirin promotes cell growth and osteogenic differentiation in murine bone marrow stromal cells》 作者:MiDu,WanPan,XiaoqiDuan,PishanYang,ShaohuaGe 期刊:Journal of Dental Sciences 影响因子:0.619 PMID:30894990

《Akt and β-catenin contribute to TMZ resistance and EMT of MGMT negative malignant glioma cell line》 作者:Guo-zhong Yi,Ya-wei Liu,Wei Xiang,Hai Wang,Zi-yang Chen,Si-di Xie,Song-tao Qi 期刊:Journal of the Neurological Sciences 影响因子:2.448 PMID:27423571

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《MicroRNA-378-3p/5p suppresses migration and invasion of oral squamous carcinoma cells by inhibiting KLK4 expression》 作者:Zhi Cui, Shiqun Sun, Qilin Liu, Xuechun Zhou, Siyu Gao, Peixuan Peng, Qianpeng Li 期刊:Biochemistry and Cell Biology 影响因子:2.014 PMID:31265790

《The downregulation of choline kinase alpha inhibits clear cell renal cell carcinoma proliferation and metastasis via the MAPK and PI3K/AKT pathways》 作者:Feng-Shi Zhang, Yang Hong, Zhen-Jie Zhu, Li-Zhe An, Xiao-Bo Huang, Qing-Quan Xu 期刊:Int J Clin Exp Med 影响因子:0.181 PMID:

《miR?210?3p regulates cell growth and affects cisplatin sensitivity in human ovarian cancer cells via targeting E2F3》 作者:Yue Jin, Jun Wei, Shaoting Xu, Fang Guan, Lijun Yin ,Haibin Zhu 期刊:Molecular Medicine Reports 影响因子:1.851 PMID:30957179

《RIP3 inhibition protects locomotion function through ameliorating mitochondrial antioxidative capacity after spinal cord injury》 作者:Yang Wang,Jianhang Jiao,Shanyong Zhang,Changjun Zheng,Minfei Wu 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:31146112

《Erythropoietin helix B surface peptide modulates miR-21/Atg12 axis to alleviates cardiomyocyte hypoxia-reoxygenation injury》 作者:Song Huang, Yongluan Lin, Zhanbo Liang, Zhuomin Wu,Yequn Chen and Chang Chen 期刊:American Journal of Translational Research 影响因子:3.266 PMID:31105848

《Examination of Alzheimer's disease by a combination of electrostatic force and mechanical measurement》 作者:W. ZHAO  W. CUI  S. XU  L.‐Z. CHEONG  C. SHEN 期刊:Journal of Microscopy 影响因子:1.813 PMID:31038737

《Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the expression of Gadd45b in cell cycle pathway》 作者:Zhongrui Yan, Fengyi Guo, Qing Yuan, Yu Shao, Yedan Zhang, Huiyan Wang, Shaohua Hao and Xue Du 期刊:Stem Cell Research & Therapy 影响因子:4.627 PMID:30606243

《ApoM-S1P Modulates Ox-LDL-Induced Inflammation Through the PI3K/Akt Signaling Pathway in HUVECs》 作者:Zhi Zheng,Yongzhi Zeng,Xiao Zhu,Ying Tan,Yi Li,Qian Li,Guanghui Yi 期刊:Inflammation 影响因子:2.939 PMID:30377890

《The expression of melatonin receptors MT1 and MT2 is regulated by E2 in sheep oviduct》 作者:Jun-jie Hu,Long-fei Xiao,Liang-li Song,Wen-bo Ge,Hong-wei Duan,Yuting Jiang 期刊:General and Comparative Endocrinology 影响因子:2.445 PMID:30849411

《E2F1 mediates the downregulation of POLD1 in replicative senescence》 作者:Shichao Gao,Qiao Song,Jing Liu,Xiaomin Zhang,Xunming Ji,Peichang Wang 期刊:Cellular and Molecular Life Sciences 影响因子:7.014 PMID:30895337

《Synergistic protection of matrine and lycopene against lipopolysaccharide?induced acute lung injury in mice》 作者:Wu?Wei Li, Tao?Yuan Wang ,Bo Cao, Bin Liu, Yu?Mei Rong ,Juan?Juan Wang ,Fei Wei ,Lu?Qing Wei, Hong Chen ,Yan?Xia Liu 期刊:Molecular Medicine Reports 影响因子:1.851 PMID:31180535

《A thermosensitive RGD-modified hydroxybutyl chitosan hydrogel as a 3D scaffold for BMSCs culture on keloid treatment》 作者:Congcong,Qu,ZixianBao,Xin,Zhang,Zhiguo,Wang,Jizhen,Ren,Zhongzheng,Zhou,Meiping Tian,Xiaojie Cheng,Xiguang Chen,Chao Feng 期刊:International Journal of Biological Macromolecules 影响因子:4.784 PMID:30529347

《Protective effects of higenamine combined with [6]-gingerol against doxorubicin-induced mitochondrial dysfunction and toxicity in H9c2 cells and potential mechanisms》 作者:Jianxia Wen,Jian Wang,Pengyan Li,Ruilin Wang,Jiabo Wang,Xuelin Zhou,Lu Zhang,Haotian Li,Shizhang Wei,Huadan Cai,Yanling Zhao 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:31028997

《Expression of inositol?requiring enzyme 1β is downregulated in azoxymethane/dextran sulfate sodium?induced mouse colonic tumors》 作者:Faliang Dai, Shizhen Dong, Zhenhua Rong, Qingxia Xuan, Pan Chen ,Menglu Chen, Yonggang Fan, Qiang Gao 期刊:Experimental and Therapeutic Medicine 影响因子:1.448 PMID:30603916

《C14orf159 suppresses gastric cancer cells’ invasion and proliferation by inactivating ERK signaling》 作者:Yan-Mei Zhu,Qiang Li, Xiao-Zhuo Gao, Xiao Meng, Li-Li Sun,Yu Shi,En-Tian Lu and Yong Zhang 期刊:Cancer Management and Research 影响因子:2.243 PMID:30863180

《Unfolded Protein Response Differentially Regulates TLR4-Induced Cytokine Expression in Distinct Macrophage Populations》 作者:Lei Zhang, Paul G. Pavicic Jr., Shyamasree Datta, Qiaoling Song, Xiaohan Xu, Wei Wei, Fan Su, Patricia A. Rayman, Chenyang Zhao and Thomas Hamilton 期刊:Frontiers in Immunology 影响因子:4.716 PMID:31293572

《miR?27b?3p and miR?607 cooperatively regulate BLM gene expression by directly targeting the 3'?UTR in PC3 cells》 作者:Yinglian Chen, Jiafu Zhao ,Zhiqiang Duan ,Ting Gong ,Wei Chen, Sainan Wang ,Houqiang Xu 期刊:Molecular Medicine Reports 影响因子:1.851 PMID:30957187

《Toll-like receptor 4 is necessary for glucose-dependent glucagon-like peptide-1 secretion in male mice》 作者:Lijuan Wang,Xiandong Zhan,Zhenhui Wang,Jing Ma,Xiaotong Chang,Xiaobo Zhu 期刊:Biochemical and Biophysical Research Communications 影响因子:2.705 PMID:30661789

《Homeobox A10 promotes the proliferation and invasion of bladder cancer cells via regulation of matrix metalloproteinase?3》 作者:Chunlei Liu, Mingzhu Ge, Jun Ma, Yanhui Zhang, Yanhui Zhao, Tao Cui 期刊:Oncology Letters 影响因子:1.871 PMID:31289471

《Carnosine Protects Mouse Podocytes from High Glucose Induced Apoptosis through PI3K/AKT and Nrf2 Pathways》 作者:Kunxiao Zhao,Ying Li, Ziqiang Wang, Ning Han, and Ying Wang 期刊:BioMed Research International 影响因子:2.197 PMID:31275971

《Glycyrrhetinic Acid Improves Insulin-Response Pathway by Regulating the Balance between the Ras/MAPK and PI3K/Akt Pathways》 作者:Yuan Zhang, Shengnan Yang, Man Zhang, Zhihua Wang, Xin He, Yuanyuan Hou and Gang Bai 期刊:Nutrients 影响因子:4.171 PMID:30871060

《A novel ELISA for the detection of active form of plasminogen activator inhibitor-1 based on a highly specific trapping agent》 作者:Le Shang,Guangpu Xue,Lihu Gong,Yaxin Zhang,Shuangzhou Peng,Cai Yuan,Mingdong Huang 期刊:Analytica Chimica Acta 影响因子:5.256 PMID:30712574








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