DMEM(低糖,含双抗) 31600
  • 品牌:Solarbio
  • 产地:中国
  • 货号:31600
  • 价格: ¥询价
  • 发布日期: 2019-11-29
  • 更新日期: 2024-04-24
产品详请
产地 中国
品牌 Solarbio
货号 31600
用途范围 细胞培养
纯度 现询客服%
规格 500ml
是否进口

产品简介 :

  1.中文名:DMEM(L) (含双抗)

  2.  号:31600-500

  3.  牌:solarbio

  4.规格:500ml

5.储存条件:4℃

6.保质期:1年

产品用途:细胞培养试剂


DMEM是现今细胞培养中比较常见的培养基之一,dmem培养基是建立在MEM培养基的基础上研究出来的,它与MEM培养基相比较,DMEM培养基中加大了MEM培养基中各种成分的用量,下文主要介绍一下自己配制DMEM的方法。

1 制备新鲜三蒸水或Millipore超纯水。

2 称取所需量的干粉培养基,加入约终体积一半的三蒸水中;若配制一个包装的培养液,在将整个包装的干粉倒入三蒸水后,需用水洗包装袋内面2次,倒入培养液中,以保证所有干粉都溶解成培养液。磁力搅拌或人工搅拌使之完全溶解。

3 根据包装袋上的要求补加所需量的碳酸氢钠;根据实验需要,添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物质。

4 加水定容到终体积。

5 必要时用1 mol/L 盐酸和1 mol/L 氢氧化钠调节pH。

6 用无菌0.22um滤膜过滤除菌,分装于无菌血清瓶中,4℃冰箱保存。

配制好的培养液用前加入100U/mL青霉素和100U/mL链霉素,并根据需要加入血清(5%-20%)。 DMEM培养基应用广泛。基于DMEM培养基中氨基酸、微生物含量丰富,并具有糖酵解中的丙酮酸和微量Fe离子,因此在细胞培养、疫苗生产等方面的应用最为常见。


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公司简介:

     北京索莱宝科技有限公司 始创于 2000年, 是一家集 产品研发、生产、销售、服务于一体的大型高科技生物企业。公司总部位于北京,拥有1200平米研发生产中心,公司提供的产品近万种,超过5000种常备现货,涵盖分子生物学、细胞生物学、免疫学、生物医学等相关领域,并不断推陈新出扩充种类,为用户提供优秀,专业的产品。  立足北京,辐射全国,随着产品营销战略的不断延伸,我们将逐步发展成为专业性生物科技公司。我们希望能够和尊敬的客户一起,利用各自的资源优势和勇于进取的敬业精神,为中国生命科学研究的发展作出更多的贡献。为了更好、更全面的发展,现公司正在诚招各地代理商,欢迎垂询并期待您的加入!

北京索莱宝科技有限公司,供应细胞培养相关产品以及其他生化试剂,实验、科研用品,参看如下产品类目,欢迎咨询!

产品类目:

1.细胞培养试剂,2.细胞培养耗材,3.蛋白质提取,4.蛋白质纯化,5.蛋白质定量,6.蛋白质电泳,7.蛋白质Marker,8.Western blot,9.免疫组化,10.抗体,11.SABC试剂盒,12.基因组和质粒提取试剂盒,13.核酸提取相关试剂,14.DNA电泳,15.DNA Marker,16.RNA相关试剂,17.PCR,18.Southern blot,19.基因工程,20.生物培养,21.碱基与核酸,22.氨基酸,23.多肽及蛋白质,24.,25.辅酶,26.酶抑制剂,27.维生素,28.抗生素,29.染色剂,30.糖与碳水化合物,31.生物缓冲液,32.植物生长激素,33.酸和盐分离效,34.,35.层析介质,36.常用生化试剂,37.其他生化试剂,38.耗材,39.标准品,40.新增产品

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