测定意义：

线粒体复合体Ⅲ（EC 1.10.2.2）又称CoQ-细胞色素C 还原酶，广泛存在于动物、植物、微生物和培养细胞的线粒体中，是线粒体呼吸电子传递链主路和支路的共有成分，负责把还原型CoQ 的氢传递给细胞色素C，生成还原型细胞色素C。
测定原理：

与氧化型细胞色素C 不同，还原型细胞色素C 在550nm 有特征光吸收，因此550nm 光吸收增加速率能够反映线粒体复合体Ⅲ酶活性。
试验中所需的仪器和试剂：

紫外分光光度计、台式离心机、水浴锅、可调式移液器、1mL玻璃比色皿、研钵、冰和蒸馏水                       

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