4',6-二脒基-2-苯基吲哚 DAPI D8200
  • 品牌:Solarbio
  • 产地:北京
  • 货号:D8200
  • cas:28718-90-3
  • 价格: ¥询价
  • 发布日期: 2019-12-19
  • 更新日期: 2024-03-29
产品详请
产地 北京
品牌 Solarbio
货号 D8200
用途范围 DNA链荧光染色
英文名称 DAPI,4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)
纯度 HPLC≥90%%
CAS编号 28718-90-3
规格 10mg
保质期 3年
是否进口
有效期3年
溶解性1 mg/mL in water
别名2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
英文名称DAPI,4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)
CAS28718-90-3
分子式C16H15N5·2HCl
分子量350.25
储存条件2-8℃
危险代码R:36/37/38 S:26-36
纯度HPLC≥90%
外观(性状)黄色粉末
单位

DNA链荧光染色。


DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB(ethidium bromide)相比,对双链DNA的染色灵敏度要高很多倍。

DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。

DAPI的*激发波长为340nm,*发射波长为488nm;DAPI和双链DNA结合后,*激发波长为364nm,*发射波长为454nm。

本DAPI溶液用水配制,加热有助于溶解。

用于细胞核染色时,推荐的DAPI工作浓度为0.5-10μg/ml。

运输与保存方法
常温运输。粉末在室温下稳定,需避光储存。
注意事项
1)DAPI对人体有一定刺激性,请注意适当防护。
2)荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。
3)为减缓荧光淬灭可以使用抗荧光淬灭封片液。
4)为了您的安全和健康,请穿实验服并戴一次性手套操作。
配制母液
用双蒸水溶解,终浓度为1mg/ml。本产品不可以用缓冲液直接溶解。-20℃可保存1年,建议分装保存,避免反复冻融。
使用方法
1.配制工作液:用双蒸水或PBS稀释母液,配制成所需要的工作的浓度(0.5-10μg/ml)。
2.固定的细胞或组织染色:
对于固定的细胞或组织样品,固定后,适当洗涤去除固定剂。DAPI染色通常在其他染色的*进行。如果不需要进行其它染色,则直接进行DAPI 染色。
a)对于贴壁细胞或组织切片:加入适量DAPI染色液,覆盖住样品即可。
对于悬浮细胞:至少加入待测染色样品体积3倍的染色液,混匀。室温放置3-5分钟。
b)吸除DAPI染色液,用TBST、PBS或生理盐水洗涤2-3次,每次3-5分钟。
c)直接在荧光显微镜下观察或封片后荧光显微镜下观察。激发波长360nm,发射波长460nm。
3. 活细胞或组织染色:
a)细胞培养物中加入适量DAPI染色液,约1/10细胞培养基体积,必须充分覆盖住待染色的样品。通常对于六孔板一个孔需加入1ml染色液,对于96孔板一个孔需加入100μl染色液。
b)在 37℃培养细胞 10~20 分钟。
c)用 PBS或合适的缓冲液洗细胞两次。

d)直接在荧光显微镜下观察或封片后荧光显微镜下观察。激发波长360nm,发射波长460nm。

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