Caspase-3活性测定试剂盒 BC3830 细胞凋亡检测
  • 品牌:Solarbio
  • 产地:北京
  • 货号:BC3830
  • 价格: ¥询价
  • 发布日期: 2019-12-16
  • 更新日期: 2024-03-19
产品详请
产地 北京
品牌 Solarbio
货号 BC3830
用途 细胞凋亡检测
产品规格 20T
纯度 现询客服%
是否进口

Caspase-3是凋亡过程中最主要的终末剪切酶,因而也是研究最多的caspase,它激活pro-caspase-2,6,7,9等,特异水解多种凋亡关键蛋白如PARP,介导细胞核染色质固缩、凋亡小体生成、核DNA断裂。试剂盒测定原理基于Caspase-3特异水解多肽底物acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA),释放硝基苯胺pNA。游离硝基苯胺pNA呈黄色在405 nm具有*吸收峰,用普通可见光分光光度比色方法测定。根据pNA标准管吸光度OD值和标准曲线,计算来自底物肽释放的pNA浓度,得到Caspase-3水解活性。反应体系100微升,试剂盒提供的试剂,除标准曲线外可进行50或100次样品测定。

适用:测定哺乳动物组织、细胞caspase-3活性。

组成与储存:
1. 裂解缓冲液: 20 ml ,4 oC
2. 反应缓冲液: 10 ml ,4 oC
3. 2 mM Ac-DEVD-pNA底物: 0.5 ml,-20 oC避光
4. 10 mM pNA标准品: 0.5 ml,-20 oC避光
以上标出的量为100次检测,50次检测的试剂量减半。试剂常温运输。到达后按要求储存,1年内稳定。

所需仪器:
酶标仪与96孔酶标板;或可见光分光光度计配100μl容积的石英比色杯。
工作波长:*吸收波长405 nm,如不具备也可在400~450 nm范围内测定。

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