|
| 产地 | 北京 |
| 品牌 | solarbio |
| 货号 | K200001M |
| 保存条件 | Store at -20°C. Avoid freeze / thaw cycles. |
| 用途 | 蛋白研究，分析，检测 |
| 应用范围 | WB IP IF |
| 抗原来源 | |
| CAS编号 | |
| 保质期 | |
| 抗体名 | Flag tag |
| 是否单克隆 | 是 |
| 克隆性 | |
| 靶点 | Flag tag |
| 适应物种 | |
| 形态 | 液体 |
| 宿主 | mouse |
| 标记物 | |
| 包装规格 | 50ul |
| 纯度 | % |
| 亚型 | IgG1 |
| 标识物 | |
| 浓度 | Flag tag% |
| 免疫原 | A synthetic peptide coupled to KLH |
| 是否进口 | 否 |
英文名称 Anti-Flag tag Monoclonal Antibody
宿主 Mouse
别名 DDDDK Tag;DYKDDDDK Tag
应用 WB IP IF
稀释比例 WB 1:10000-15000. IP 1:100-300. IF 1:500-3000.
保存 Store at -20°C. Avoid freeze / thaw cycles.
储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
纯化方法 Affinity purification
亚型 IgG1
免疫原 A synthetic peptide coupled to KLH
性状 液体
Public Immunogen Range A synthetic peptide coupled to KLH
*号 2C5
*类型 Monoclonal Antibody
背景资料 The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an .. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence.
英文名称 Anti-Flag tag Monoclonal Antibody
宿主 Mouse
别名 DDDDK Tag;DYKDDDDK Tag
应用 WB IP IF
稀释比例 WB 1:10000-15000. IP 1:100-300. IF 1:500-3000.
保存 Store at -20°C. Avoid freeze / thaw cycles.
储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
纯化方法 Affinity purification
亚型 IgG1
免疫原 A synthetic peptide coupled to KLH
性状 液体
Public Immunogen Range A synthetic peptide coupled to KLH
*号 2C5
*类型 Monoclonal Antibody
背景资料 The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an .. IF there is no against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an against the Flag sequence.
