英文名称 Anti-GAPDH Monoclonal antibody

宿主 Mouse

别名 GAPDH

应用 WB IHC IF

稀释比例 WB 1:3000-8000. IHC 1:100-300. IF 1:100-200.

交叉反应 Human Mouse Rat Monkey Chicken Rabbit Hamster

蛋白分子量 36kDa

Gene ID 2597

保存 Store at -20°C. Avoid freeze / thaw cycles.

储存液 Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.

纯化方法 Affinity purification

亚型 IgG1

免疫原 Recombinant protein of human GAPDH

性状 液体

Public Immunogen Range Recombinant protein of human GAPDH

Subcellular Locations Cytoskeleton Cytosol Nucleus

Swiss Prot P04406

*类型 Monoclonal Antibody

背景资料 Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer's and Huntington's disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

参考文献

《β-N-Oxalyl-L-α,β-diaminopropionic acid from Panax notoginseng plays a major role in the treatment of type 2 diabetic nephropathy》 作者:Li Jie,Qiu Pengcheng,Wang Siwang,WuJun sheng,He Qiaoyan,Li Kaifeng,Xu Lu 期刊:biomedicine & pharmacotherapy 影响因子:3.743 PMID:30928803