产品说明：

苏木精-伊红染色法 ( Hematoxylin-Eosin staining )，简称 HE 染色法 ，是病理学常规制片中最基本的染色方法。苏木精染液为碱性 ，主要使细胞核内的染色质与胞质内的核糖体着紫蓝色；伊红为酸性染料，主要使细胞质和细胞外基质中的成分着红色 。染色过程需要优化，着色情况与组织或细胞的种类有关，也随其生活周期及病理变化而改变。例如，很多细胞在新生时期胞浆对伊红着色较淡或轻度嗜碱，当其衰老时或发生退行性变则呈现嗜伊红浓染。胶原纤维在老化和出现透明变性时，伊红着色由浅变深。本产品所包含试剂均为工作液，可直接使用。

操作说明（以下步骤仅供参考）：

（一）石蜡组织切片染色。

1．取材组织块，经固定后，常规石蜡包埋，切片。

2．石蜡切片脱蜡水化：

①二甲苯（I）中脱蜡 5min。

②换用新鲜的二甲苯（Ⅱ），再脱蜡 5min。

③无水乙醇 5 min。

④95%乙醇 2min。

⑤80％乙醇 2min

⑥70%乙醇 2min。

⑦蒸馏水 2min。

3．苏木素染液染色 5-20min(具体时间根据染色结果和实验要求调整)，自来水冲洗。

4．分化液分化 30s。

产品内容 G1120-10 G1120-100

苏木素染液 10ml 100ml

分化液 10ml 100ml

伊红染液 10ml 100ml

5．自来水浸泡 15min 或温水（约 50℃）5min。

6．置伊红染液几秒-2min(具体时间根据染色结果和实验要求调整)，自来水冲洗。

7．自来水浸泡 2-5min。

8．脱水，透明，封片：

①95％乙醇（I）2-3s

②95％乙醇（Ⅱ）2-3s

③100％乙醇（I）2-3s

④100％乙醇（Ⅱ）1min

⑤二甲苯石炭酸（3：1）1min

⑥二甲苯（I）1min

⑦二甲苯（Ⅱ）1min

⑧中性树胶封固，镜下观察。

（二）冰冻切片

冰冻切片不用脱蜡，可固定后直接染色，其方法与石蜡切片相同。

注意事项：

1、切片脱蜡应尽量干净。

2、系列乙醇应经常更换新液。

3、第一次使用本试剂盒时建议先取 1-2 个样品做预实验。

4、染色过程推荐浅染，通常只需能够分辨细胞核即可，颜色过深有可能影响细胞质颜色。

5、冷冻切片染色时间尽量要短。

6、 为了您的安全和健康，请穿实验服并戴一次性手套操作。

相关产品：

G1010 姬姆萨染色液

G1040 瑞氏染色液

G1100 伊红染色液

G1140 苏木素染色液（常规染色）

P2100 10×多聚赖氨酸

产品说明：  

           苏木精   -  伊红染色法    ( Hematoxylin-Eosin staining )  ，简称   HE  染色法       ，切片技术里常用的染色法之一       。苏木精染液为碱性       ，主要使细胞核内的染色质与胞质内的核糖体着紫蓝色；伊红为酸性染料，主要使细胞质和细胞外基质中的成分着红色   。

           染色过程需要优化，着色情况与组织或细胞的种类有关，也随其生活周期及病理变化而改变。例如，很多细胞在新生时期胞浆对伊红着色较淡或轻度嗜碱，当其衰老时或发生退行性变则呈现嗜伊红浓染。胶原纤维在老化和出现透明变性时，伊红着色由浅变深。

          本产品所包含试剂均为工作液，可直接使用。

 G1020 瑞氏-姬姆萨复合染液 100ml/500ml 220/800  

 G1040 瑞氏染色液 50ml/100ml 100/160  

 G1060 革兰氏染色液(试剂盒) 4×(10/100/500)ml 80/400/1200  

 G1061 结晶紫染色液（2.5%） 10/100/500ml 40/120/400  

 G1062 结晶紫染色液（1%） 10/100/500ml 40/120/400  

 G1063 结晶紫染色液（0.1%） 10/100/500ml 40/120/400  

 G1066 卢戈碘液（革兰氏染色） 10/100/500ml 40/120/400  

 G1067 番红染色液（沙黄） 10/100/500ml 40/120/400  

 G1069 卢戈碘液（标准碘液） 100ml/500ml 120/400  

 G1080 Mayer'苏木素染液(免疫组化) 10ml/100ml/500ml 60/320/1400  

 G1100 伊红染色液 100ml/500ml 160/480  

 G1120 HE染色液(试剂盒) 3×10ml/3×100ml 140/400  

 G1130 荚膜染色液（试剂盒） 2*50/2*100/2*250ml 160/260/520  

 G1132 芽孢染色液（试剂盒） 2*50/2*100/2*250ml 160/260/520  

 G1134 鞭毛染色液（试剂盒） 100ml 240  

 G1142 Weigert苏木素染色液 2*50/2*100/2*500ml 140/210/620  

 G1140 Cole氏苏木素染色液(常规染色) 100ml/500ml 210/620  

 G1160 石炭酸复红染液(苯酚品红染液) 100ml/500ml 160/500  

 G1165 改良型石炭酸复红染液(苯酚品红染液) 100ml/500ml 200/800  

 G1170 抗酸染色液（试剂盒） 3*50/3*100/3*250ml 200/300/600  

 G1180 0.1%吕氏碱性美蓝染色液 100ml/500ml 160/500  

 G1185 0.4%吕氏碱性美蓝染色液 100ml/500ml 160/500  

 G1330 Van Gieson 染色液(VG染色液) 50/100/500ml 60/100/300  

 G1333 Van Gieson 染色液(VG染色液，含苏木素) 3*50/3*100/3*500ml 180/300/900  

 G1210 溴甲酚绿-甲基红指示剂溶液 100ml/500ml 100/260  

 G1220 甲苯胺蓝溶液 100ml/500ml 140/400  

 G1260 饱和油红O染色液 100ml/500ml 100/260  

 G1270 亮绿SF染色液（1%） 100ml/500ml 120/400  

 G1310 中性红染色液(0.33% 过滤除菌，活细胞染色) 100ml/500ml 200/600   

 G1315 中性红染色液(0.5% 细胞或组织切片染色) 100ml/500ml 160/500  

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