测定意义

辅酶ⅠNAD(H)广泛存在于动物、植物、微生物和培养细胞中，NAD⁺是糖酵解（EMP）和三羧酸循环（TCA）的主要氢受体，生成的NADH经呼吸电子链（ETC）传递把电子交给氧，在合成ATP的同时，形成大量的ROS，同时NADH再生为NAD⁺。糖、脂、蛋白质三大代谢物质分解中的氧化反应绝大部分通过这一体系完成。NAD(H)含量和NADH/NAD⁺比值的高低可用于评价糖酵解和TCA循环的强弱。较高的NAD(H)及NADH/NAD⁺比值说明细胞呼吸耗氧量较高，处于过氧化状态。此外，NADH/NAD⁺比值升高也可抑制糖酵解和TCA循环。另外，NAD⁺降解产物对细胞信号传导、代谢和基因表达等具有重要的调控作用。

测定原理

分别用酸性和碱性提取液提取样品中NAD⁺和NADH，NADH通过PMS的递氢作用，还原氧化型噻唑蓝（MTT）为甲瓒，在570nm下检测吸光值；而NAD⁺可被乙醇脱氢酶还原为NADH，进一步采用MTT还原法检测。

相关文献：

《Understanding the stress responses of Kluyveromyces marxianus after an arrest during high-temperature ethanol fermentation based on integration of RNA-Seq and metabolite data》 作者:Xiaofen Fu， Pengsong Li，Lei Zhang， Shizhong Li 期刊:Applied Microbiology and Biotechnology 影响因子:3.67 PMID:30673809
《α-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPT/NAD Controlled Inflammatory Reactions》 作者:Mengqing Tao,1Jia Jiang,Lin Wang,Yan Li, Qingcheng Mao, Jiyang Dong, and Jian Zuo 期刊:Evidence-Based Complementary and Alternative Medicine 影响因子:1.984 PMID:30405740
《FK866 inhibits the epithelial?mesenchymal transition of hepatocarcinoma MHCC97?H cells》 作者:Bin Zhang Dongmei Shi Xiangyu Zhang Guanzhao Liang Weida Liu Sen Qiao 期刊:Oncology Letters 影响因子:1.871 PMID:
《Involvement of the two l-lactate dehydrogenase in development and pathogenicity in Fusarium graminearum》 作者:Wenchan ChenLingling WeiYu ZhangDongya ShiWeichao RenZhihui ZhangJin WangWenyong ShaoXiali LiuChangjun ChenEmail authorQingli Gao 期刊:Curr Genet 影响因子:3.464 PMID:30474697
《Modulating acetate ester and higher alcohol production in Saccharomyces cerevisiae through the cofactor engineering》 作者:Kun-Qiang Hong,Xiao-Meng Fu,Sheng-Sheng Dong,Dong-guang Xiao,Jian Dong 期刊:J. Ind. Microbiol. Biotechnol. 影响因子:2.993 PMID:30969383
《Metabolomics Analysis Reveals Therapeutic Effects of α-Mangostin on Collagen-Induced Arthritis in Rats by Down-regulating Nicotinamide Phosphoribosyltransferase》 作者:Kui Yang,Qin Yin,Qingcheng Mao,Sheng Dai,Lin Wang,Jiyang Dong,Jian Zuo 期刊:Inflammation 影响因子:2.939 PMID:30484004