产品简介 

  1.中文名：MTT溶液（0.5%）

  2.英文名：MTT

  3.分  类：生化试剂

  4.保存条件：RT

  5.品  牌：solarbio

MTT溶液（0.5%）的详细描述

    MTT溶液（0.5%）仅用于科研试验。

MTT溶液（0.5） 染色液

MTT是一种检测细胞存活和生长的方法。MTT实验原理为活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT还原为水不溶性的蓝紫色结晶甲瓒（Formazan）并沉积在细胞中，而死细胞无此功能。二甲基亚砜（DMSO）能溶解细胞中的甲瓒，用酶联免疫检测仪在490nm波长处测定其光吸收值，可间接反映活细胞数量。
在一定细胞数范围内，MTT结晶形成的量与细胞数成正比。根据测得的吸光度值(OD值)，来判断活细胞数量，OD值越大，细胞活性越强(如果是测药物毒性，则表示药物毒性越小)。该方法已广泛用于一些生物活性因子的活性检测、大规模的抗肿瘤药物筛选、细胞毒性试验以及肿瘤放射敏感性测定等。它的特点是灵敏度高、经济。
MTT通常用于.细胞增殖及细胞活性测定以及药物(也包括其他处理方式如放射线照射)对体外培养的细胞毒性的测定。
缺点：由于MTT经还原所产生的甲瓒产物不溶于水，需被溶解后才能检测。这不仅使工作量增加，也会对实验结果的准确性产生影响，而且溶解甲的有机溶剂对实验者也有损害。
注意事项：
1、在配制和保存的过程中，容器用铝箔纸包住。
2、配成的MTT需要无菌，MTT对菌很敏感
3、MTT一般现用现配，过滤后4度避光保存两周内(个人曾做过4度避光保存4周的MTT溶液，效果仍然不错)有效，或配制成5mg/ml保存在-20度长期保存，避免反复冻融，小剂量分装，用避光袋或是黑纸、锡箔纸包住避光以免分解。当MTT变为灰绿色时就*不能再用。
4、MTT有致癌性，用的时候小心,有条件带那种透明的簿膜手套.
通常，此法中的MTT浓度为5mg/ml。因此，可以称取MTT 0.5克，溶于100 ml的磷酸缓冲液（PBS）或无酚红的培养基中，用0.22μm滤膜过滤以除去溶液里的细菌，放4℃ 避光保存即可。在配制和保存的过程中，容器用铝箔纸包住。

MTT溶液（0.5） 染色液

 
参考文献：
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《Carbon dots serve as an effective probe for the quantitative determination and for intracellular imaging of mercury(II)》 作者:Fanyong Yan,Depeng Kong,Yunmei Luo,Qianghua Ye,Juanjuan He,Xingfei Guo,Li Chen 期刊:Microchimica Acta 影响因子:5.705 PMID:
《LncRNA SNHG15 acts as a ceRNA to regulate YAP1-Hippo signaling pathway by sponging miR-200a-3p in papillary thyroid carcinoma》 作者:DM Wu, S Wang, X Wen, XR Han, YJ Wang 期刊:Cell death & Disease 影响因子:8.371 PMID:30237435
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《Effect of cell culture models on the evaluation of anticancer activity and mechanism analysis of the potential bioactive compound, iturin A, produced by Bacillus subtilis》 作者:H Zhao, X Zhao, S Lei, Y Zhang, D Shao, C Jiang 期刊:Food & function 影响因子:3.289 PMID:30778489
《IL-17A/IL-17RA promotes invasion and activates MMP-2 and MMP-9 expression via p38 MAPK signaling pathway in non-small cell lung cancer》 作者:Zhenhua Wu,Dan He,Shuyuan Zhao,Hongjiang Wang 期刊:Molecular and cellular biochemistry 影响因子:3.062 PMID:30564960
《SIRT1 expression regulates the transformation of resistant esophageal cancer cells via the epithelial-mesenchymal transition》 作者:Tiantian Qin,Weihua Liu,Junfeng Huo,Leilei Li,Xueyan Zhang,Xiaoli Shi,Jinlei Zhou,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影响因子:2.068 PMID:29656187
《Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells》 作者:Shengnan Guo,Yuanyuan Wang, Yu Gao,Yinxu Zhang, Mingzi Chen, Minghao Xu, Lu Hu, Yu Jing, Fangyu Jing, Chen Li, Qingjun Wang, and Zhitu Zhu 期刊:International Medical Journal of Experimental 影响因子:1.42 PMID:27774979
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《Excitation-independent yellow-fluorescent nitrogen-doped carbon nanodots for biological imaging and paper-based sensing》 作者:Lihong Shi,Lin Li,Xiaofeng Li,Guomei Zhang,Yan Zhang,Chuan Dong,Shaomin Shuang, 期刊:Sensors and Actuators B: Chemical 影响因子:5.667 PMID:
《Nanodiamond-conjugated transferrin as chemotherapeutic drug delivery》 作者:Zhiqin Wang,Zhimei Tian,Yu Dong,Lin Li,Lu Tian,Yingqi Li,Binsheng Yang 期刊:Diamond and Related Materials 影响因子:2.232 PMID:24998271
《Toxicity of imidazoles ionic liquid [C16mim]Cl to Hela cells》 作者:Xiaohua Xi,Ruyan Wan,Peijin Wang,Weiran Huo,Hui Dong,Qiyan Du 期刊:Ecotoxicology and Environmental Safety 影响因子:3.974 PMID:30015186
《线粒体靶向抗氧化剂MitoQ 的抗肿瘤效应》 作者:王华伟， 王华南， 康肖梦， 张宇睿， 杨福军… 期刊:辐射研究与辐射工艺 影响因子:0.471 PMID:
《RNAi构建parp-1基因低表达的石英恶性转化细胞模型》 作者:郭伟， 宋珊珊， 田琳， 高艾， 牛丕业， 左昕 期刊:首都医科大学学报 影响因子:1.511 PMID:
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《Simultaneous determination of saikosaponins in Bupleurum Radix from different locations by high performance liquid chromatography- charged aerosol detection (HPLC-CAD) method and its immunomodulation effects on mouse splenocytes》 作者:Lina Wang, Bin Chen, Wei Wang, Nan Xu and Tianzhu Jia* 期刊:academic Journals 影响因子: PMID:
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《Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes》 作者:Shicheng Bi, Xiaodan Ma, Yuemin Wang, Xiaoqing Chi, Yong Zhang, Wei Xu, and Songhua Hu 期刊:Oxidative Medicine and Cellular Longevity 影响因子:4.868 PMID:31178974
《The role and mechanism of transforming growth factor beta 3 in human myocardial infarction‐induced myocardial fibrosis》 作者:Ke Xue , Jun Zhang , Cong Li , Jing Li , Cong Wang,  Qingqing Zhang,  Xianlu Chen , Xiaotang Yu , Lei Sun ,Xiao Yu 期刊:Journal of Cellular and Molecular Medicine 影响因子:4.658 PMID:30983140
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《Curcumin analogues attenuate Aβ25-35-induced oxidative stress in PC12 cells via Keap1/Nrf2/HO-1 signaling pathways》 作者:Jialin Xu，Leilei Zhou，QiWeng，Linxia Xiao，Qingyong Li 期刊:Chemico-Biological Interactions 影响因子:3.407 PMID:30946834
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《Scutellarin protects human retinal pigment epithelial cells against hydrogen peroxide (H2O2)-induced oxidative damage》 作者:Xin Hu, Xiaofang Wu, Bo Zhao and Yongyi Wang 期刊:Cell & Bioscience 影响因子:3.355 PMID:30680088
《Andrographolide prevents human nucleus pulposus cells against degeneration by inhibiting the NF‐κB pathway》 作者:Jianwei Liu , Tongmeng Jiang,  Mingwei He , Depeng Fang,  Chong Shen,  Yiguan Le , Maolin He , Jinmin Zhao,  Li Zheng 期刊:Journal of cellular physiology 影响因子:4.522 PMID:30370694
《Honokiol inhibits breast cancer cell metastasis by blocking EMT through modulation of Snail/Slug protein translation》 作者:Wen-die Wang, Yue Shang, Yi Li & Shu-zhen Chen 期刊:Acta Pharmacologica Sinica 影响因子:4.01 PMID:31235819
《Effects of nanoparticle size on the interaction between zinc oxide nanoparticles and bovine serum albumin》 作者:Liwei Wang, Tingting Liang, Jun Ma, Liqian Sun, Congcong Yang, Liqiang Meng et al. 期刊:Journal of Biomolecular Structure and Dynamics 影响因子:3.31 PMID:30938584
《NADPH Oxidase Isoforms Are Involved in Glucocorticoid-Induced Preosteoblast Apoptosis》 作者:Shu-Cai Bai,Qian Xu, Hui Li, Ya-Fei Qin, Li-Cheng Song, Chen-Guang Wang, Wen-Hao Cui, Zhi Zheng,De-Wen Yan,Zhi-Jun Li, Dong Li, Xin Wan,and Hua-Feng Zhang 期刊:Oxidative Medicine and Cellular Longevity 影响因子:4.868 PMID:31049140
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