产品目录
当前位置 : 首页 > 染色剂 > 4',6-二脒基-2-苯基吲哚 DAPI D8200
4',6-二脒基-2-苯基吲哚 DAPI D8200
起批量( 价格
≥1 ¥800元/瓶
  • 供货能力:1000
  • 最低订购量:1瓶
  • 可销售总数量:1000瓶
  • 建议零销价:800元/瓶
  • 发布日期: 2019-12-19
  • 更新日期: 2019-12-19
产品详细说明
品牌: Solarbio CAS编号: 28718-90-3
产地: 北京 英文名称: DAPI,4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)
保质期: 3年 货号: D8200
含量: HPLC≥90% 用途范围: DNA链荧光染色
规格: 10mg 是否进口:
有效期3年
溶解性1 mg/mL in water
别名2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride
英文名称DAPI,4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI)
CAS28718-90-3
分子式C16H15N5·2HCl
分子量350.25
储存条件2-8℃
危险代码R:36/37/38 S:26-36
纯度HPLC≥90%
外观(性状)黄色粉末
单位

DNA链荧光染色。


DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB(ethidium bromide)相比,对双链DNA的染色灵敏度要高很多倍。

DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。

DAPI的*激发波长为340nm,*发射波长为488nm;DAPI和双链DNA结合后,*激发波长为364nm,*发射波长为454nm。

本DAPI溶液用水配制,加热有助于溶解。

用于细胞核染色时,推荐的DAPI工作浓度为0.5-10μg/ml。

运输与保存方法
常温运输。粉末在室温下稳定,需避光储存。
注意事项
1)DAPI对人体有一定刺激性,请注意适当防护。
2)荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。
3)为减缓荧光淬灭可以使用抗荧光淬灭封片液。
4)为了您的安全和健康,请穿实验服并戴一次性手套操作。
配制母液
用双蒸水溶解,终浓度为1mg/ml。本产品不可以用缓冲液直接溶解。-20℃可保存1年,建议分装保存,避免反复冻融。
使用方法
1.配制工作液:用双蒸水或PBS稀释母液,配制成所需要的工作的浓度(0.5-10μg/ml)。
2.固定的细胞或组织染色:
对于固定的细胞或组织样品,固定后,适当洗涤去除固定剂。DAPI染色通常在其他染色的*进行。如果不需要进行其它染色,则直接进行DAPI 染色。
a)对于贴壁细胞或组织切片:加入适量DAPI染色液,覆盖住样品即可。
对于悬浮细胞:至少加入待测染色样品体积3倍的染色液,混匀。室温放置3-5分钟。
b)吸除DAPI染色液,用TBST、PBS或生理盐水洗涤2-3次,每次3-5分钟。
c)直接在荧光显微镜下观察或封片后荧光显微镜下观察。激发波长360nm,发射波长460nm。
3. 活细胞或组织染色:
a)细胞培养物中加入适量DAPI染色液,约1/10细胞培养基体积,必须充分覆盖住待染色的样品。通常对于六孔板一个孔需加入1ml染色液,对于96孔板一个孔需加入100μl染色液。
b)在 37℃培养细胞 10~20 分钟。
c)用 PBS或合适的缓冲液洗细胞两次。

d)直接在荧光显微镜下观察或封片后荧光显微镜下观察。激发波长360nm,发射波长460nm。

相关文献:

《Hematopoietic Substrate-1-Associated Protein X-1 Regulates the Proliferation and Apoptosis of Endothelial Progenitor Cells Through Akt Pathway Modulation》 作者:XIN-BIN GUO,XIN DENG,YING WEI 期刊:Wiley Online Library 影响因子: PMID:29139175
《Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A》 作者:Lingjuan Du, Guojian Li, Yong Yang, Guokai Yang, Jia Wan, Zhenhuan Ma, Yi Hou 期刊:International Journal of Clinical and Experimental Pathology 影响因子:0.205 PMID:
《Stem cells from human exfoliated deciduous teeth ameliorate type II diabetic mellitus in Goto-Kakizaki rats》 作者:Nanquan Rao, Xiaotong Wang, Yue Zhai, Jingzhi Li, Jing Xie, Yuming Zhao and Lihong Ge 期刊:Diabetology & Metabolic Syndrome 影响因子:2.361 PMID:30858895
《The treatment of systematically transplanted gingival mesenchymal stem cells in periodontitis in mice》 作者:Wendong Sun, Zhiguo Wang, Quanchen Xu, Huibin Sun, Xiaoxuan Liu, Jianjun Yang, Rundan Hong 期刊:Experimental and Therapeutic Medicine 影响因子:1.448 PMID:30783482
《Macropinocytosis-dependent endocytosis of Japanese flounder IgM+ B cells and its regulation by CD22》 作者:Yiqun Li,Li Sun,Jun Li 期刊:Fish & Shellfish Immunology 影响因子:3.298 PMID:30266605
《Nobiletin inhibits Il-1?-induced inflammation in chondrocytes via suppression of NF-kB signaling and attenuates osteoarthritis in mice》 作者:Zeng Lin, Dengying Wu, Lipeng Huang, Chao Jiang, Tianlong Pan, Xiaodiao Kang and Jun Pan 期刊:Frontiers in Pharmacology 影响因子:3.845 PMID:31214026
《Escin suppresses HMGB1‐induced overexpression of aquaporin‐1 and increased permeability in endothelial cells》 作者:Changjun Chen, Songgang Wang, Jiying Chen, Xiaolin Liu, Mengchen Zhang, Xi Wang, Weihua Xu,Yayun Zhang, Hao Li, Xin Pan, Meng Si 期刊:FEBS Open Bio homepage 影响因子:1.959 PMID:30972964
《Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture》 作者:Yi-Meng Duan, Ying Jin, Meng-Li Guo, Leng-Xin Duan, and Jian-Gang Wang 期刊:Journal of Cancer 影响因子:3.182 PMID:30087713
《Strontium/adiponectin co-decoration modulates the osteogenic activity of nano-morphologic polyetheretherketone implant》 作者:ShengnanWang,YuanyiYang,YunfeiLi,JiachengShi,JianshuZhou,LiZhang,YiDeng,WeizhongYang 期刊:Colloids and Surfaces B:Biointerfaces 影响因子:3.997 PMID:30592990
《A Host Factor GPNMB Restricts Porcine Circovirus Type 2 (PCV2) Replication and Interacts With PCV2 ORF5 Protein》 作者:Kangkang Guo, Lei Xu, Mengmeng Wu, Yufeng Hou, Yanfen Jiang, Jiangman Lv, Panpan Xu, Zhixin Fan, Ruiqi Zhang, Fushan Xing and Yanming Zhang 期刊:Frontiers in Microbiology 影响因子:4.019 PMID:30671053
《Quantitative Evaluations of a Novel Detergent-Based Method for Decellularization of Human Cervical Carcinoma Tissues》 作者:Pengpeng Xiao, Jicheng Han, Ying Zhang, Chenghui Li, Xiaofang Guo, Shubo Wen, Mingyao Tian, Yiquan Li, Maopeng Wang, Hao Liu, Jingqiang Ren, Hongning Zhou,Huijun Lu and Ningyi Jin, 期刊:Cellular and Infection Microbiology 影响因子: PMID:
《The effects of fluoride on neuronal function occurs via cytoskeleton damage and decreased signal transmission》 作者:Lingli Chen,Hongmei Ning,Zhihong Yin,Xiaochao Song,Yongchao Feng,Hao Qin,Yi Li,Jundong Wang,Yaming Ge,Wenkui Wang 期刊:Chemosphere 影响因子:4.427 PMID:28719878
《Biopanning of mouse bone marrow mesenchymal stem cell affinity for cyclic peptides》 作者:Guozong Wang, Zhentao Man, Nianping Zhang, Hua Xin, Yi Li, Tiantong Sun, Shui Sun 期刊:Molecular Medicine Reports 影响因子:1.922 PMID:30431079
《Mechanically cartilage-mimicking poly(PCL-PTHF urethane)/collagen nanofibers induce chondrogenesis by blocking NF–kappa B signaling pathway》 作者:T Jiang, D Kai, S Liu, X Huang, S Heng, J Zhao 期刊:Biomaterials 影响因子:7.604 PMID:29945065
《Multiwall carbon nanotubes/polycaprolactone composites for bone tissue engineering application》 作者:Lanlan Pan,Xibo Pei,Rui He,Qianbing Wan,Jian Wang 期刊:Colloids and Surfaces B: Biointerfaces 影响因子:3.554 PMID:22305638
《S‐Allylmercaptocysteine Attenuates Cisplatin‐Induced Nephrotoxicity through Suppression of Apoptosis, Oxidative Stress, and Inflammation》 作者:Xiaosong Zhu , Xiaoyan Jiang , Ang Li , Zhongxi Zhao OrcID and Siying Li 期刊:nutrients 影响因子:4.196 PMID:
《Ras-association domain family 1 (RASSF1A) gene regulates progression, migration and invasion of bladder cancer》 作者:Yuhai Bao,Xuefeng Liu,Yang Liu,Si Wang,Bin Wu 期刊:Surgical Oncology 影响因子:2.136 PMID:
《Nell-1 promotes the neural-like differentiation of dental pulp cells》 作者:Qi Han,Qiang Wang,Jiameng Wu,Mengyue Li,Yixuan Fang,Hongfan Zhu,Xiaoying Wang 期刊:Biochemical and Biophysical Research Communications 影响因子:2.705 PMID:30979495
《Helicobacter pylori infection promotes epithelial-to-mesenchymal transition of gastric cells by upregulating LAPTM4B》 作者:Shengfei Zhou,Hui Chen,Peihua Yuan,Ning Shi,Xiao Wang,Jinxia Hu,Luying Liu 期刊:Biochemical and Biophysical Research Communications 影响因子:2.705 PMID:31084933
《Generation of bioartificial hearts using decellularized scaffolds and mixed cells》 作者:Cailing Tong, Cheng Li, Baiyi Xie, Minghui Li, Xianguo Li, Zhongquan QiEmail author and Junjie Xia 期刊:BioMedical Engineering OnLine 影响因子:2.013 PMID:31164131
《Erythropoietin helix B surface peptide modulates miR-21/Atg12 axis to alleviates cardiomyocyte hypoxia-reoxygenation injury》 作者:Song Huang, Yongluan Lin, Zhanbo Liang, Zhuomin Wu,Yequn Chen and Chang Chen 期刊:American Journal of Translational Research 影响因子:3.266 PMID:31105848
《Examination of Alzheimer's disease by a combination of electrostatic force and mechanical measurement》 作者:W. ZHAO  W. CUI  S. XU  L.‐Z. CHEONG  C. SHEN 期刊:Journal of Microscopy 影响因子:1.813 PMID:31038737
《microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9》 作者:Yang Xu, Yan Huang, Yibing Guo, Yicheng Xiong, Shajun Zhu, Liancheng Xu, Jingjing Lu, Xiaohong Li, Jian Wan, Yuhua Luand Zhiwei Wang 期刊:Stem Cell Research & Therapy 影响因子:4.627 PMID:30767782
《Effect of photobiomodulation on neural differentiation of human umbilical cord mesenchymal stem cells》 作者:Hongli Chen,Hongjun Wu,Huijuan Yin,Jinhai Wang,Huajiang Dong ,Qianqian Chen,Yingxin Li 期刊:Lasers in Medical Science 影响因子:2.076 PMID:30232645
《The Damaging Effects of Pedunsaponin A on Pomacea canaliculata Hemocytes》 作者:Chunping Yang , Tianxing Lv ,Bin Wang,Xiaoyan Qiu,Liya Luo ,Min Zhang , Guizhou Yue , Guangwei Qin , Deshan Xie and Huabao Chen 期刊:Toxins 影响因子:3.895 PMID:31277361
《ApoM-S1P Modulates Ox-LDL-Induced Inflammation Through the PI3K/Akt Signaling Pathway in HUVECs》 作者:Zhi Zheng,Yongzhi Zeng,Xiao Zhu,Ying Tan,Yi Li,Qian Li,Guanghui Yi 期刊:Inflammation 影响因子:2.939 PMID:30377890
《The expression of melatonin receptors MT1 and MT2 is regulated by E2 in sheep oviduct》 作者:Jun-jie Hu,Long-fei Xiao,Liang-li Song,Wen-bo Ge,Hong-wei Duan,Yuting Jiang 期刊:General and Comparative Endocrinology 影响因子:2.445 PMID:30849411
《The lncRNA SNHG16 affects prognosis in hepatocellular carcinoma by regulating p62 expression》 作者:Jian‐Hong Zhong , Xiao Xiang , Yan‐Yan Wang,  Xu Liu,  Lu‐Nan Qi , Cheng‐Piao Luo,  Wen‐E Wei, Xue‐Mei You , Liang Ma , Bang‐De Xiang  Le‐Qun Li 期刊:Journal of Cellular Physiology 影响因子:4.522 PMID:31256427
《lncRNA Gm10451 regulates PTIP to facilitate iPSCs-derived β-like cell differentiation by targeting miR-338-3p as a ceRNA》 作者:Yan Huang,Yang Xu, Yuhua Lu,Shajun Zhu,Yibing Guo,Cheng, Sun,Lianchen Xu ,Xiaolan Chen,Yahong Zhao,Bin Yu,Yumin Yang,Zhiwei Wang 期刊:Biomaterials 影响因子:10.273 PMID:31220795
《The role and mechanism of transforming growth factor beta 3 in human myocardial infarction‐induced myocardial fibrosis》 作者:Ke Xue , Jun Zhang , Cong Li , Jing Li , Cong Wang,  Qingqing Zhang,  Xianlu Chen , Xiaotang Yu , Lei Sun ,Xiao Yu 期刊:Journal of Cellular and Molecular Medicine 影响因子:4.658 PMID:30983140
《Cadmium induces actin cytoskeleton alterations and dysfunction in Neuro‐2a cells》 作者:Yaming Ge , Xiaochao Song,  Lingli Chen , Dongfang Hu,  Liushuai Hua , Yunli Cui,  Junwei Liu , Zhixing An, Zhihong Yin,  Hongmei Ning 期刊:Environmental Toxicology 影响因子:2.649 PMID:30614199
《Zoledronic acid inhibits osteoclast differentiation and function through the regulation of NF-κB and JNK signalling pathways》 作者:Xiao?Lin Huang, Lie?Yu Huang, Yu?Ting Cheng ,Fang Li ,Qian Zhou ,Chao Wu, Qian?Hui Shi, Zhi?Zhong Guan ,Jian Liao ,Wei Hong 期刊:International Journal of Molecular Medicine 影响因子:2.928 PMID:31173157
《Monosialoganglioside protects against bupivacaine-induced neurotoxicity caused by endoplasmic reticulum stress in rats》 作者:Benquan Liu,Jiemei Ji, Qing Feng, Xi Luo, Xiurong Yan, Yuxia Ni, Yajun He, Zhongxuan Mao and Jingchen Liu 期刊:Drug Design, Development and Therapy 影响因子:3.208 PMID:30858700
《Cucurbit[7]uril enhances photosensitization of porphyrins in Neuroblastoma cells》 作者:Xiaoyu Li,Bo Xiao,Yan Guo,Yan Xiao,Song Xiao 期刊:Photodiagnosis and Photodynamic Therapy 影响因子:2.589 PMID:30648639
《Halloysite nanotubes coated 3D printed PLA pattern for guiding human mesenchymal stem cells (hMSCs) orientation》 作者:Fan Wu,Jingqi Zheng,Zhixiong Li,Mingxian Liu 期刊:Chemical Engineering Journal 影响因子:8.355 PMID:
《CFLAR is a critical regulator of cerebral ischaemia-reperfusion injury through regulating inflammation and endoplasmic reticulum (ER) stress》 作者:Xiaohong Wang,Jun Zhao,Hongmei Guo,Qinqin Fan 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:



网易彩票

·