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ANNEXIN V-FITC/PI凋亡检测试剂盒 CA1020
起批量(20T 价格
≥1 ¥680元/20T
  • 供货能力:1000
  • 最低订购量:120T
  • 可销售总数量:100020T
  • 建议零销价:680元/20T
  • 发布日期: 2019-12-18
  • 更新日期: 2020-03-05
产品详细说明
品牌: solarbio CAS编号:
产地: 北京 货号: CA1020
是否进口: 纯度: 现询客服 %
用途: 细胞凋亡是细胞的基本特征之一,在机体的胚胎发育、组织修复、内环境的稳定等方面都起到十分重要的作用。 产品规格: 20T/50T/100T

ANNEXIN V- FITC/PI 凋亡检测试剂盒.








产品说明:

细胞凋亡是细胞的基本特征之一,在机体的胚胎发育、组织修复、内环境的稳定等方面都起到 十分重要的作用。Annexin V 是一种分子量为 35.8 KD 的 Ca2+依赖性磷脂结合蛋白,能与细胞凋亡 过程中翻转到膜外的磷脂酰丝氨酸(Phosphatidylserine, PS)高亲和力特异结合。FITC-Annexin V 结 合到凋亡细胞后,在蓝色光的激发下,发出绿色荧光,区分出凋亡细胞与正常细胞。碘化丙啶 (Propidium iodide,PI)是一种核酸染料,它不能穿透完整细胞膜,但对凋亡晚期细胞和死细胞的 破损细胞膜能够穿透,并使细胞核红染。将 FITC-Annexin V 与PI 匹配使用,可以将凋亡早期的细 胞和晚期的细胞区分开来。 :

操作步骤
1、 用 27ml 的去离子水稀释 3ml Binding Buffer(10×)至 30ml,每次用 3ml。
2、 收集细胞(1×106 个/次),然后用冷的 PBS 洗涤。(对于贴壁细胞,先用胰酶消化,再用 PBS 洗 涤)。
3、 用 1ml 1×的 Binding Buffer 悬浮细胞,300×g 离心 10min,弃上清。
4、 用 1ml 1×的 Binding Buffer 重悬细胞,使细胞的密度达到 1×106 个/ml。
5、 每管加入 100μL 细胞(1×105 个)。
6、 再向管中加入 5μL Annexin V-FITC。
7、 室温,避光,轻轻地混匀,10min。
8、 加入 5μL PI,室温,避光,孵育 5min。
9、 加入 PBS 至 500μL,轻轻混匀。
10、在 1 小时内用流式细胞仪检测。

注意事项
1、旋帽离心管装的试剂在开盖前请短暂离心,将盖内壁上的液体甩至管底,避免开盖时液体洒落。
2、Annexin V-FITC 和 Propidium iodide 是光敏物质,在保存与操作时请注意避光。
3、Propidium iodide(PI)能通过皮肤吸收,对眼睛有刺激作用。
4、在细胞洗涤的*一步,请尽量将上清弃净,以免 PBS 残留影响实验结果。 产品内容: CA1020-20 CA1020-50 CA1020-100 保存 Annexin V-FITC 100μl 250μl 500μl 4°C,避光,不要冷冻 Propidium iodide(PI) 100μl 250μl 500μl 4°C,避光,不要冷冻 Binding Buffer(10×) 6ml 15ml 30ml 4°C 保存,长时间-20°C
5、PI 染色时间过长有可能造成检测的凋亡率偏高,建议首先进行 Annexin V-FITC 染色,上机前 5 分钟再加入PI 染色。
6、整个操作过程动作要尽量轻柔,勿用力吹打细胞,尽量在 4℃下操作。
7、反应完毕后请尽快检测,因为细胞凋亡是一个动态的过程,反应 1 小时后荧光强度就开始衰变。
8、成功的检测凋亡受以下几种因素的影响,如细胞类型、细胞膜上 PS 的密度、发生凋亡时 PS 翻 转的比例、诱导细胞凋亡的方法、所用试剂、诱导凋亡的时间等,把这些影响因素进行优化对实验 成功是非常必要的。 此试剂盒仅供科研使用。


相关产品:

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CA1120   细胞凋亡-Hoechst染色试剂盒

T2190    TUNEL细胞凋亡检测试剂盒

CA1030   Annexin V PE/7-AAD 凋亡检测试剂盒

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《Mechanism of miR-15a regulating the growth and apoptosis of human knee joint chondrocytes by targeting SMAD2》 作者:Tengjun Ma, Yan Cheng & Liang Tan 期刊:Artif Cells Nanomed Biotechnol 影响因子:4.462 PMID:31366242

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