产品简介 

  1.中文名：DAPI溶液（1mg/ml）

  2.英文名：DAPI

  3.别  名：4',6-二脒基-2-苯基吲哚

  4.货  号：C0060

  5.品  牌：solarbio

  6.储存条件：-20℃，有效期至少 2 年 

  7.用  途 : 产品说明： 

    DAPI(4’,6-二脒基-2-苯基吲哚*)是一种能够与 DNA 中大部分 A，T 碱基相互结合的荧光染料﹐常用于荧光显微镜观测。因为 DAPI 可以透过完整的细胞膜﹐它可以用于活细胞和固定细胞的染色。当 DAPI 与双链 DNA 结合时，大吸收波长为 358nm，大发射波长为 461nm。DAPI 的发射光为蓝色，且 DAPI 和绿色荧光蛋白 GFP 或 Texas Red 染剂(红色荧光染剂)的发射波长仅有少部分重叠，可以利用这项特性在单一的样品上进行多重荧光染色。 本产品为 DAPI 水溶液，纯度≥90%，浓度为 1mg/ml。使用时根据实验不同直接将本产品用相应溶液稀释到工作浓度。  

使用方法： （仅供参考） 

z  对于培养细胞 

1， 取适量 DAPI 水溶液加到 PBS 中，制备成 5-15 μg/ml 的 DAPI 溶液。 

2， 将 1/10 培养基体积的 DAPI 溶液加入到细胞培养基中。 

3， 在 37℃培养细胞 10-20 分钟。 

4， 用 PBS 或合适的缓冲液洗细胞两次。 

5， 置于荧光显微镜下观察，激发波长 360-400nm。 

z  对于组织切片 制好的玻片上滴加几滴稀释的 DAPI 染液，染色 10 分钟，流水冲去染液，滤纸吸除多余水分，加一滴荧光封片液，置于荧光显微镜下观察。 

注意事项： 

 DAPI 被普遍认为具有致癌性，操作时应戴手套，并避免交叉污染。 

     本产品需避光，并尽量避免反复冻融。 

相关产品： 

 C0080  碘化丙锭 PI溶液（1mg/ml） 

 CA1020 ANNEXIN V-FITC/PI凋亡检测试剂盒 

 12100 DMEM(H)培养基 

 S9000  特级胎牛血清  

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